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71.
A procedure for the production of conjugates of soybean peroxidase (SbP) oxidized by sodium periodate and anti-mouse IgG antibody (Ab) was optimized. A sandwich chemiluminescent enzyme-linked immunosorbent assay (ELISA) for determination of mouse IgG using SbP and specific Ab was developed, and SbP-catalyzed oxidation of luminol was carried out in the absence of any enhancer. Comparison of conjugates produced by labeling Ab by soybean and horseradish peroxidases in the chemiluminescent ELISA showed that in the case of SbP a rate of emission decay formed through luminol oxidation was significantly lower. Application of the soya enzyme allowed the development of the immunoassay with improved sensitivity and a wider linear range. 相似文献
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Winfried Schröder Stefan Nickel Simon Schönrock Roman Schmalfuß Werner Wosniok Michaela Meyer Harry Harmens Marina V. Frontasyeva Renate Alber Julia Aleksiayenak Lambe Barandovski Oleg Blum Alejo Carballeira Maria Dam Helena Danielsson Ludwig De Temmermann Anatoly M. Dunaev Barbara Godzik Katrin Hoydal Zvonka Jeran Gunilla Pihl Karlsson Pranvera Lazo Sebastien Leblond Jussi Lindroos Siiri Liiv Sigurður H. Magnússon Blanka Mankovska Encarnación Núñez-Olivera Juha Piispanen Jarmo Poikolainen Ion V. Popescu Flora Qarri Jesus Miguel Santamaria Mitja Skudnik Zdravko Špirić Trajce Stafilov Eiliv Steinnes Claudia Stihi Ivan Suchara Lotti Thöni Hilde Thelle Uggerud Harald G. Zechmeister 《Annals of Forest Science》2017,74(2):31
Key message
Moss surveys provide spatially dense data on environmental concentrations of heavy metals and nitrogen which, together with other biomonitoring and modelling data, can be used for indicating deposition to terrestrial ecosystems and related effects across time and areas of different spatial extension.Context
For enhancing the spatial resolution of measuring and mapping atmospheric deposition by technical devices and by modelling, moss is used complementarily as bio-monitor.Aims
This paper investigated whether nitrogen and heavy metal concentrations derived by biomonitoring of atmospheric deposition are statistically meaningful in terms of compliance with minimum sample size across several spatial levels (objective 1), whether this is also true in terms of geostatistical criteria such as spatial auto-correlation and, by this, estimated values for unsampled locations (objective 2) and whether moss indicates atmospheric deposition in a similar way as modelled deposition, tree foliage and natural surface soil at the European and country level, and whether they indicate site-specific variance due to canopy drip (objective 3).Methods
Data from modelling and biomonitoring atmospheric deposition were statistically analysed by means of minimum sample size calculation, by geostatistics as well as by bivariate correlation analyses and by multivariate correlation analyses using the Classification and Regression Tree approach and the Random Forests method.Results
It was found that the compliance of measurements with the minimum sample size varies by spatial scale and element measured. For unsampled locations, estimation could be derived. Statistically significant correlations between concentrations of heavy metals and nitrogen in moss and modelled atmospheric deposition, and concentrations in leaves, needles and soil were found. Significant influence of canopy drip on nitrogen concentration in moss was proven.Conclusion
Moss surveys should complement modelled atmospheric deposition data as well as other biomonitoring approaches and offer a great potential for various terrestrial monitoring programmes dealing with exposure and effects.75.
Electrosurgery reduces blood loss and immediate postoperative inflammation compared to cold instruments for midline celiotomy in dogs: A randomized controlled trial 下载免费PDF全文
76.
Tomo M. Milosevic Ivan P. Glisic Nebojsa T. Milosevic Ivana S. Glisic 《European journal of plant pathology / European Foundation for Plant Pathology》2010,126(1):73-79
During 2005–2007, under the environmental conditions of Cacak (43°53’N; 20°21’E), western Serbia, the effect of Plum pox virus (PPV) on the phenology of vegetative shoot and fruit growth was investigated in a plum orchard of cv. ‘Cacanska Rodna’ including
trees non-infected with PPV and those with infection and clearly visible symptoms, as verified by RT-PCR. The results showed
that PPV did not affect the growth phenology of the vegetative shoot (length and thickness) and the fruit growth. However,
it caused negative effects on the average length (22.31 ± 0.28 cm in non-infected trees, 18.35 ± 0.07 cm in infected trees)
and thickness (4.21 ± 0.03 mm in non-infected trees, 4.01 ± 0.02 mm in infected trees) of vegetative shoots as well as on
the average fruit weight (20.43 ± 0.16 g in non-infected fruits, 17.58 ± 0.18 g in infected fruits) and fruit dimensions.
The greatest effect of PPV during the trial was the induction of a massive premature fruit drop and, consequently, a 52.94%
decrease in total yields per tree (18.0 ± 1.10 kg) and unit area (12.0 ± 0.89 t ha-1) in infected vs non-infected trees. 相似文献
77.
Jean Guyot Virgile Condina Fabien Doaré Christian Cilas Ivan Sache 《European journal of plant pathology / European Foundation for Plant Pathology》2010,126(3):349-362
South American leaf blight (SALB) is a severe threat to world rubber production. One way of controlling it is to set up plantations
in zones not conducive to the disease. Such zones are known once a plantation has been set up, but few data are available
on how climate affects the disease, especially in the Amazon region. With better knowledge of conditions that are favourable
to SALB epidemics it would be possible to more accurately identify risk zones in Asia and Africa, continents that are still
SALB-free. Based on a trial design involving detailed and frequent observations, and with a method rarely used in plant epidemiology,
the segmentation method, the results presented in this article make it possible to list, in order of importance, climatic
factors that influence disease severity under conditions where the climate varies little over the year. 相似文献
78.
Yong Zhang Qichao Song Jun Yan Jianwei Tang Rongrong Zhao Yueqiang Zhang Zhonghu He Chunqin Zou Ivan Ortiz-Monasterio 《Euphytica》2010,174(3):303-313
Investigations on concentration of mineral elements including Fe and Zn in wheat grains are important for human health. Two
hundreds and sixty-five cultivars and advanced lines were collected and sown at Anyang experimental station of the Institute
of Crop Science of the Chinese Academy of Agriculture Sciences in season 2005–2006 to evaluate the genetic variation of major
mineral element concentrations in wheat grain. Twenty-four selected cultivars were also planted at seven representative locations
in seasons 2005–2006 and 2006–2007 to evaluate the effects of genotype, environment, and genotype by environment interaction
on mineral element concentrations. The 265 genotypes displayed a large variation for all mineral elements investigated including
Fe and Zn, ranging from 28.0 to 65.4 mg kg−1 and 21.4 to 58.2 mg kg−1 for Fe and Zn, with mean values of 39.2 and 32.3 mg kg−1, respectively. Jimai 26, Henong 326, and Jingdong 8 displayed high Fe and Zn concentrations, and Jimai 26 and Henong 326
also displayed high concentrations of Cu, Mg, K, P, and protein content. Jingdong 8 is the most promising leading cultivar
for increasing Fe and Zn concentrations. All mineral element concentrations including Fe and Zn were largely influenced by
environment effects. Production of high Fe concentration can be best secured at Jiaozuo and Jinan, and high Zn concentration
can be best secured at Jinan and Xuzhou, since samples from these locations in the two seasons are characterized by high Fe
or Zn concentration, compared with the other locations. High and significant genotype by environment interaction effects on
all mineral element concentrations were also observed, with ratios of genotype by environment to genotype variances all larger
than 1.20. Grain Fe concentration was highly significant and positively correlated with that of Zn, indicating a high possibility
to combine high Fe and Zn traits in wheat breeding. It also indicated strong positive correlations between concentrations
of Fe, Zn, and protein content. 相似文献
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